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After thoroughly mixing the extract by vortexing, the precipitated protein was removed by centrifugation (16,000 g , 5 min, 4 C) before the supernatants were analysed by HPLCMS
Exendin-(9-39) has been found to competitively bind to the human GLP-1 receptor ( in vivo in humans ( in vivo that endogenous GLP-1 plays an important role on maintaining glucose homeostasis both in the fasting and in the postprandial state through its effects on pancreatic islets
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